Bioassays are one of the few analytical tools where native proteins can be characterized in vitro, under physiological conditions. They are essential in assuring integrity and functionality of a biological product. Product-specific set of bioanalytical procedures is developed for each product to monitor critical control points.
We offer a range of immuno and cell based assays to enable the drug development of your compound: from pre-clinical and clinical phases to lot release and stability testing. We have experience with a wide array of biologic products including monoclonal antibodies, antibody fragments, multi-specifics (Bites, Tribody, Bibodies, Bispecific IgGs), fusions, biosimilars, cytokines and growth factors.
Rodon can offer testing for product specificity utilizing Enzyme Linked Immunosorbent Assays (ELISA). It is the most common method for ligand binding assays due to its fast, reliable and cost-effective technique. This assay is a core competency for our team for analyte quantification as well as for determine affinity and cross-reactivity against other non-specific targets.
Cell-based assays provide a functional read-out of compound activity or produce results that are more biologically relevant. Rodon's experienced team is able to test a large variety of different cell types:
• Primary cells
• Immortalized/transformed cell lines
• Commercially available immortalized/transformed cell lines
• Whole blood
• Isolated human blood cells (PBMC, lymphocytes, monocytes, etc.)
• Customized transfected cell lines
Our expertise includes the setting of a wide range of cellular assay services either by both implementing client assays and developing them de novo. At Rodon these types of bioassays are implemented and carried out on a routine basis in biosafety level 2 laboratory facilities, against suitable controls, and wherever possible International Reference Standards.
Bioassays include, but are not limited to:
• Ligand/Receptor Binding
• Cell Proliferation Assays
• Cytotoxicity/Survival/Apoptosis (e.g. ADCC, T-cell mediated cytotoxicity)
• Cellular Differentiation
• Reporter Assays
• Cytokine Induction
• Biomarkers expression
• Immune cell activation and proliferation
From early discovery through translation to in vivo pharmacology, Rodon experience team will define the right combination of cell-based assays as your product move through the drug development life cycle, screening not only for potency and selectivity while minimizing toxicity and off-target binding.
We Offer More
Looking for additional services? We offer even more services than listed, contact us to find out how we can assist you. Using latest advances in Protein Technologies Rodon Biologics strives to provide the highest quality custom protein services in the industry. We work closely with you, analyze your project at the planning stage and take several approaches to ensure you get the results you are looking for.Know More