Cell Line Development

Rodon has extensive experience with both microbial (E.coli, yeast) and mammalian cell expression and culture. Using our proprietary and in-licensed technology, proteins can be rapidly tested in different constructs, to identify the most suitable system. using tightly regulated inducible promoters for microbial expression and  CHO platforms for mammalian expression as well as metabolic engineering of cell lines to produce small molecules.

Mammalian cell line development is based on a simple workflow which allows obtaining a high producing single cell clone in 16-20 weeks. Moreover, once a high producing pool is generated, the quality of the material is evaluated and it can be used to produce gram per liter quantities of material in 9-12 weeks.

The stability of the cell line generated is evaluated and using this technology, even after 60 generations, the expression level of the stable clone and genetic stability  is maintained.

Our services include:
• Plasmid construction with the gene of interest
• Cell transfection with antibiotic selection
• Pool selection and amplification (cell banking)
• Establish clonal CHO cell lines with documented evidence of clonality
• Establish research cell banks (RCB) and study their stability (up to 6 generations without selection)
• Execute and develop thorough analytics and specific assay development support