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Protein Production - Rodon Biologics

Protein Production

Rodon Biologics provides small to large-scale protein expression and purification. Our protein production capabilities range from parallel screening of several constructs at small scale through process development optimization to manufacturing.

Starting from gene sequence, recombinant vectors, hybridoma cells or stable cell lines, we can produce recombinant proteins to support your initial research and pre-clinical studies. Worked with different proteins such as antibodies, antibody fragments, multi-specifics (Bites, Tribody, Bibodies, Bispecific IgGs), monovalent IgGs, fusions, cytokines, growth factors, hard-to-produce proteins, Rodon developed strategies for different expression systems. We can use our platform production process, execute your protocol, or customize a production process to meet your yield and quality requirements.

Our services include:
• Molecular Cloning
- Vector construction
- Customising - e.g., inclusion of tags for purification, insertion of protease signal for tag removal, signal peptides for secretion

• Protein Expression
- Expression systems: Bacteria (E. coli); Yeast (Sacchromyces cerevisiae, Pichia pastoris), Mammalian cells (CHO and HEK293)
- Transient production: from mg-gram quantities in shake flasks and bioreactors (10L working volume)
- Stable engineered cell lines: grams per liter in fed batch processes

• Protein Purification and Isolation
- Purification of soluble proteins (cytoplasm, periplasm, secreted)
- Purification of insoluble proteins from inclusion bodies, including refolding
- Chromatography (affinity, Ion-X, HIC, MMC, RP, membrane)
- Filtration (Depht, Micro, X-floe, viral)

• Quality Control
- Assays for concentration (A280, Dye biding assay and HPLC)
- Assays for purity (HPLC, gel electrophoresis, Western Blot, Endotoxin level and microbiological tests)
- ELISA and product specific functional assays
- Stability testing

We Offer More

Looking for additional services? We offer even more services than listed, contact us to find out how we can assist you. Using latest advances in Protein Technologies Rodon Biologics strives to provide the highest quality custom protein services in the industry. We work closely with you, analyze your project at the planning stage and take several approaches to ensure you get the results you are looking for.

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